Book Title: Engineering Bacteriophage Laboratory

Subtitle: Biochemistry 375 Lab Manual

Author: Erica Shu

Cover image for Engineering Bacteriophage Laboratory

All Rights Reserved

Contents

Preface
Introduction to Engineering Bacteriophage Laboratory
Lab 1: Introduction to a Biochemistry Laboratory
Lab 2: PCR Amplification of the T7 Bacteriophage
Lab 3: Analysis of PCR Products
Lab 4: Preparation for YAC-Based T7 Genome Assembly in Yeast
Lab 5: YAC-Based Genome Assembly of the T7 Mutant Phage
Lab 6: Screening for Assembled T7-YAC Clones
Lab 7: Analysis of the Multiplexed PCR Products
Lab 8: Purification and Bacterial Transformation of the T7-YAC Vector
Lab 9: T7 Mutant Phage Procurement and Phage Enumeration using the Plaque Titer Assay
Lab 10: Phage Enumeration: Plaque Titer Assay Part II
Lab 11: Phage Killing Assay
Lab 12: Phage Killing Assay in Multiple Hosts
Lab 13: Microbial Population Editing Assay
Appendices
1. Appendix I: Oligo Sequences
Acknowledgements

Book Information

Author

Erica Shu

License

Engineering Bacteriophage Laboratory Copyright © 2023 by Erica Shu. All Rights Reserved.

Subject

Biochemistry

Metadata

Title: Engineering Bacteriophage Laboratory
Author: Erica Shu
License: Engineering Bacteriophage Laboratory Copyright © 2023 by Erica Shu. All Rights Reserved.
Primary Subject: Biochemistry
Additional Subject(s): Scientific research, Microbiology (non-medical)
Institution: University of Wisconsin-Madison