Lab 2 PreLab Assignments

PreLab Assignment

Complete in your notebook (part of the lab performance score). Note that you will need to review the lab protocol in detail to complete both this assignment and the quiz.

• What will you be doing in the lab and why?
• What should you end up with at the end of the lab period?
• Write down an outline of the lab procedure; bullet points, tables and figures are fine.
• Come to the lab with as many of the reaction calculations completed as possible (see the procedure that follows).

PreLab Quiz

You can take the quiz in Canvas here.

The quiz questions are also listed below for your convenience:

• Question 1 MC: You will be provided with a 5X reaction buffer stock. You want your 50 μL reaction to have a final concentration of 1X reaction buffer. How many μL of the 5X stock must you add to each reaction?
  • 3 μL
  • 10 μL
  • 5 μL
• Question 2 MC: You will be provided with a 10 mM dNTP stock. You want your 50 μL reaction to have a final concentration of 200 μM dNTP. How many μL of the dNTP stock must you add to each reaction?
  • 1 μL
  • 1 μL
  • 5 μL
• Question 3 MC: You will be provided with a 1 U/ μL Phusion enzyme stock. You want your 50 μL reaction to contain 1 U Phusion. How many μL of the Phusion stock must you add to each reaction?
  • 1 μL
  • 5 μL
  • 5 μL