Lab 5 PreLab Assignments

PreLab Assignment

Complete in your notebook (part of the lab performance score). Note that you will need to review the lab protocol in detail in order to complete both this assignment and the quiz.

• What will you be doing in the lab and why?
• What should you end up with at the end of the lab period? Where will it be located?
• Write down an outline of the lab procedure; bullet points, tables and figures are fine.

PreLab Quiz 

You can take the quiz in Canvas here.

The quiz questions are also listed below for your convenience:

• Question 1 T/F: Yeast is in the log growth phase if the culture has an OD600 of 1.2.
• Question 2 T/F: If a yeast culture has an OD600 of 1.2, one should dilute the yeast culture and remeasure the OD600.
• Question 3 MC: What is the purpose of heat shock during transformation (select all that apply)?
  • Allowing the yeast to grow
  • Increasing membrane permeability
  • Allowing the yeast to pick up DNA from its surrounding