LAB 1 MINI REPORT

LAB 1 MINI REPORT 

Reports must be typed and submitted to Canvas by the start of your next lab (see the “Course Summary” tab for links to all assignment submissions). You can find a sample Lab 1 report in the assignment submission page. Lab notes are not part of the mini lab reports and should not be submitted.

Refer to the general guidelines for Mini Lab Reports on Canvas, found here (link opens in a new tab): Lab Mini Reports

View the following video for tips on making a great PyMOL figure:

Results Section

Pick one of the three mutations you made, and address the following:

• State the residue that you changed and what amino acid you changed it to (e.g. V34K). Include a figure showing the change in the structure.Please make sure that the PyMOL figures you use in the lab report have a white background – this will make them significantly easier to evaluate. Remember that figures need to have informative titles and legends. Your legend should clearly state what is shown in each color and what is depicted as sticks/spheres/etc.
• Justify why you chose to change that amino acid and why you selected the mutant residue that you did. Use your knowledge of the structure from the PyMOL session in Lab 1 and the biochemical properties of the amino acids (Fig. 1.7 or any general biochemistry textbook) to inform your answer.
• Comment on clashes that appear with your mutated residues.
• Study the effect of the mutation you picked on the ligand-binding structure. (The ligand is dansylamide, DNSA). Does it seem compatible, or does the mutation create clashes?

Discussion Section

What effect on HCAII function do you expect to observe for the mutation? Consider the following:

• Different functionality of the new residue (is it a conservative or non-conservative mutation?)
• Effects on structure
• Effects on enzymatic activity
• Effects on ligand-binding

What is the next step? How would you test the function of the mutant?