Lab 7: Purification of His-tagged wild type and mutant HCAII using Ni Resin
Lab 7 Objectives
- Purify HCAII protein from E. coli, obtaining as high quantity and purity as possible
In lab 6, you expressed His-tagged HCAII in E. coli. In this experiment, you will purify the protein on a nickel column. One pair from each pod will purify wild-type protein while the other pair will purify the mutant protein. Once the protein has been purified and analyzed, you will share protein with the other group in your pod so that both groups have purified mutant and wild-type protein. Both groups from each pod will work together to assay both the mutant and the wild type protein in Labs 9 – 11.
