Lab 8: Analysis of His-tagged HCAII expression and purification

Lab 8 Objectives

  • Determine whether you induced expression of HCAII protein from your pETblue2-HCAII clone (during lab 6)
  • Determine whether you successfully purified HCAII on a nickel column (during lab 7)
  • Measure the protein concentration of your purified HCAII protein
Fig. 8.1. A sample SDS-PAGE gel.

License

Biochemistry 551 Lab Manual Copyright © by Lynne Prost. All Rights Reserved.