Contents

  1. Biochemistry 551: Biochemical Methods, Online Version

  2. Lab 1: Structural analysis of HCAII using PyMOL

    1. Lab 1: Structural Analysis of HCAII using PyMOL

    2. Lab 1: BACKGROUND

    3. Lab 1: PROCEDURE Part 1

    4. Lab 1: PROCEDURE Part 2

    5. Lab 1: PROCEDURE Part 3

    6. Lab 1: PROCEDURE Part 4

    7. Lab 1: LAB ASSIGNMENTS

    8. Lab 1: MINI LAB REPORT

  3. Lab 2: PCR Amplification of HCAII and pETblue2

    1. Lab 2: PCR Amplification of HCAII and pETblue2

    2. Lab 2: BACKGROUND

    3. Lab 2: PROCEDURE Part 1

    4. Lab 2: PROCEDURE Part 2

    5. Lab 2: LAB ASSIGNMENTS

    6. Lab 2: MINI LAB REPORT

  4. Lab 3: Lab-at-home: Introduction to Biotechnology Methods

    1. Lab 3: Lab-at-home: Introduction to Biotechnology Methods

    2. Lab 3: BACKGROUND AND PROCEDURE

    3. Lab 3: LAB ASSIGNMENTS

    4. Lab 3: WRITE UP

  5. Lab 4: Gibson assembly of HCAII into pETblue2 vector and transformation of E. coli with the reactions

    1. Lab 4: Gibson assembly of HCAII into pETblue2 vector and transformation of E. coli with the reactions

    2. Lab 4: BACKGROUND

    3. Lab 4: PROCEDURE

    4. Lab 4: LAB ASSIGNMENTS

    5. Lab 4: MINI LAB REPORT

  6. Lab 6: Mutant Exploration

    1. Lab 6: Mutant Exploration

    2. Lab 6: BACKGROUND

    3. Lab 6: PROCEDURE

    4. Lab 6: LAB ASSIGNMENTS

    5. Lab 6: MINI LAB REPORT

  7. Lab 5: Screening for pETblue2-HCAII clones

    1. Lab 5: Screening for pETblue2-HCAII clones

    2. Lab 5: BACKGROUND

    3. Lab 5: PROCEDURE

    4. Lab 5: LAB ASSIGNMENTS

    5. Lab 5: MINI LAB REPORT

  8. Lab 7: Protein Expression and Purification in E. coli

    1. Lab 7: Protein expression and purification in E. coli

    2. Lab 7: BACKGROUND

    3. Lab 7: PROCEDURE

    4. Lab 7: LAB ASSIGNMENTS

    5. Lab 7: MINI LAB REPORT

  9. Lab 8: Analysis of His-tagged HCAII Expression and Purification

    1. Lab 8: Analysis of His-tagged HCAII expression and purification

    2. Lab 8: BACKGROUND

    3. Lab 8: PROCEDURE

    4. Lab 8: LAB ASSIGNMENTS

    5. Lab 8: DATA ANALYSIS

    6. Lab 8: MINI LAB REPORT

  10. Lab 9: Determination of Protein Stability using Chemical Denaturation and Intrinsic Fluorescence

    1. Lab 9: Determination of Protein Stability using Chemical Denaturation and Intrinsic Fluorescence

    2. Lab 9: AT-HOME EXPERIMENT

    3. Lab 9: BACKGROUND

    4. Lab 9: PROCEDURE

    5. Lab 9: LAB ASSIGNMENTS

    6. Lab 9: DATA ANALYSIS

    7. Lab 9 MINI LAB REPORT

  11. Lab 10: Investigation of wild type and mutant HCAII enzyme activity

    1. Lab 10: Investigation of the wild type and mutant HCAII enzyme activity

    2. Lab 10: AT-HOME EXPERIMENT

    3. Lab 10: BACKGROUND

    4. Lab 10: PROCEDURE

    5. Lab 10: LAB ASSIGNMENTS

    6. Lab 10: DATA ANALYSIS

    7. Lab 10: MINI LAB REPORT

  12. Lab 11: Forster Resonance Energy Transfer (FRET) to detect ligand-binding to HCAII

    1. Lab 11: FRET to detect ligand binding to HCAII

    2. Lab 11: BACKGROUND

    3. Lab 11: PROCOTOL

    4. Lab 11: LAB ASSIGNMENTS

    5. Lab 11: DATA ANALYSIS

    6. Lab 11: FINAL LAB REPORT

  13. Appendices

    1. Appendix 1: Information on HCAII

    2. Appendix 2: Statistical methods used in 551 data analyses

    3. Appendix 3: Additional laboratory details

    4. Appendix 4: Buffer formulations

    5. Appendix 5: Operation of the Shimadzu spectrophotometers

  14. Appendix