Lab 6: LAB ASSIGNMENTS

PRE-LAB QUIZ

This week’s quiz is based on the mutageneic primers needed for the QuikChange PCR. You will be given a mutation number and the primers that were used to generate the mutation. Based on this information, determine the specific amino acid change for your mutation.

IN-LAB ACTIVITIES

You and your partner will meet in a BBCU breakout room to work through the lab procedure together.

  • Work through the lab procedure and the provided Google doc, which ask you to:
    • create a protocol for QuikChange mutagenesis
    • complete a sequence alignment
    • generate a PyMOL figure
    • discuss what makes a good hypothesis and critique several sample hypotheses
    • write your own hypothesis
  • Put the required information into your slides in the shared class Google slides presentation.
  • At the designated time, you will join a TA and several other pairs to present and discuss your slides.
  • Complete the lab/partner/self evaluation Google form.
  • Get a sharable link to the Google doc and submit it to Canvas.

License

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Biochemistry 551 (Online Version) Lab Manual by Lynne PROST is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.

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