Refer to the general guidelines for Mini Lab Reports on Canvas, found here (link opens in a new tab): Mini Lab Report Guidelines


Be sure to include:

  • Purpose statement/brief summary of the experiment
  • Cite the lab manual
  • Enzyme used for digestion
  • How the DNA was purified (you DO NOT need to include the full protocol for DNA purification) and measured its concentration
  • Descriptions of the three Gibson Assembly reactions (using DNA amounts instead of volumes wherever possible) and how the reaction was run
  • Key information about the transformation: the strain of E. coli, the method of transformation (i.e. heat shock), and the antibiotic used in the plate. You do not need to include step-by-step protocol for the transformation.


Include the following information in your Results section:

  • The concentrations and purity of DNA. A table might be nice here (note that, like figures, table should have a legend; however, table legends go above the table).
  • State how many colonies resulted from each of the DNA amounts used, including the negative control. Include separate counts of blue versus white colonies. A table might be nice here.
  • Comment briefly on whether the different amounts of DNA resulted in different numbers of colonies.
  • Be careful not to add Discussion text here! Your Results should describe what you observed, not why you think you observed it.


Icon for the Creative Commons Attribution 4.0 International License

Biochemistry 551 (Online Version) Lab Manual Copyright © by Lynne PROST is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.

Share This Book