Lab 8: Analysis of His-tagged HCAII expression and purification
Lab 8 Objectives
- Use SDS-PAGE to:
- Determine expression of HCAII protein was induced inĀ E. coli
- Determine whether HCAII was successfully purified on a nickel column
- Measure the protein concentration of the final purified HCAII protein
![](https://wisc.pb.unizin.org/app/uploads/sites/502/2020/06/stained-gel.jpg)