Lab 8: LAB ASSIGNMENTS
PRELAB QUIZ
Question 1
What experiments are being simulated this week? What is the purpose of those experiments? (Provide 2-3 sentences).
Question 2
This lab involved a chemical hazard. Describe the chemical, the potential risks, and steps you would take to protect yourself.
Question 3
If you assemble your gel apparatus and find that it leaks, describe one way you can try to fix it.
Question 4
Which is likely to polymerize faster: Resolving gel (bottom, poured first), or Stacking gel (top, poured second)?
IN-LAB ACTIVITIES
- After joining BBCU, you and your partner will join a breakout room.
- Your TAs will provide you a link to a Google doc. You and your partner will work together to complete it. You will:
- Choose samples to run on your SDS-PAGE gels
- Answer questions about the procedure
- Discuss how to troubleshoot several example cases where something went wrong
- Determine the protein concentrations of the final, dialyzed protein samples
- Meet with your TAs to discuss. Once they are happy with your work, you will receive a picture of the gel you can analyze for your results.
- You are welcome to use any remaining in-lab time to analyze your gel and start working on your lab report.
- Complete the lab/partner/self evaluation Google form.
- Get a sharable link to your Google doc and submit it to Canvas.
IMPORTANT NOTE FOR LAB 9
For lab 9, you will need to complete an at-home experiment before your lab time. Please plan out your next week accordingly!
The at-home experiment requires 2-4 eggs and 1/3 cup rubbing alcohol.