Lab 5: Screening for pETblue2-HCAII clones

Lab 5 Objectives

Determine whether theĀ HCAII gene is present in pETblue2 in each of your potential clones; by:

  • Using the blue/white screening technique to identify bacteria suspected to contain your clone of interest
  • Purifying DNA from these potential clones
  • Digesting the purified DNA with restriction enzymes to differentiate positive vs. negative clones (i.e. a restriction digest screen)



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Biochemistry 551 (Online Version) Lab Manual Copyright © by Lynne PROST is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.

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