Lab 11: FRET to detect ligand binding to HCAII

Lab 11 Objectives

  • Determine the binding constant (KD) of a ligand (dansyl amide, DNSA) and of an inhibitor (acetazolamide, AZ) to wild type and mutant HCAII

 

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Biochemistry 551 (Online Version) Lab Manual by Lynne PROST is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.

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