Refer to the general guidelines for Mini Lab Reports on Canvas, found here (link opens in a new tab): Mini Lab Reports


  • Describe all the experimental conditions tested (using concentrations, not volumes)
  • Describe all the data analysis and equations you used to determine the values you report in the Results


  • For both wild type and mutant, show one representative set of fluorescence emission curves (wavelength on the x-axis, fluorescence on the y-axis, one curve per [urea]).
  • Show the Xf and ΔGunfolding plots you generated in Excel. Include error bars.
    • Note: error bars in Excel are actually kind of hard. Here is a link to some documentation that may help. You will want to do custom error bars, and be sure to use only vertical bars (not horizontal): link to Excel error bars help
    • Compare the shapes of these curves. Are the wt and mutant the same or can you observe some differences?
  • Use a table to report the CM and ΔGunfolding (as determined in Prism) for both wt and mutant protein. Be sure to include measurements of error, and state clearly what measurements you are using.
  • Report statistical significance and p-values where possible.


  • How do your CM and ΔGunfolding values compare to published values? What are some reasons why your values might differ from what has been published?
  • Point out any abnormalities in the data and provide possible explanations.
  • What did you learn about the effect of your mutation on the stability of HCAII?


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Biochemistry 551 (Online Version) Lab Manual Copyright © by Lynne PROST is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.

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