Lab 4: Gibson assembly of HCAII into pETblue2 vector and transformation of E. coli with the reactions
Lab 4 Objectives
- Prepare DNA for Gibson Assembly
- Use a Gibson Assembly reaction to clone the HCAII gene into the pETblue2 expression vector
- Transform E. coli with the cloned DNA using the heat shock method
![](https://wisc.pb.unizin.org/app/uploads/sites/502/2020/06/Gibson-overview-300x232.png)