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Biochemistry 551: Biochemical Methods, Online Version
Lab 1: Structural Analysis of HCAII using PyMOL
Lab 1: BACKGROUND
Lab 1: PROCEDURE Part 1
Lab 1: PROCEDURE Part 2
Lab 1: PROCEDURE Part 3
Lab 1: PROCEDURE Part 4
Lab 1: LAB ASSIGNMENTS
Lab 1: MINI LAB REPORT
Lab 2: PCR Amplification of HCAII and pETblue2
Lab 2: BACKGROUND
Lab 2: PROCEDURE Part 1
Lab 2: PROCEDURE Part 2
Lab 2: LAB ASSIGNMENTS
Lab 2: MINI LAB REPORT
Lab 3: Lab-at-home: Introduction to Biotechnology Methods
Lab 3: BACKGROUND AND PROCEDURE
Lab 3: LAB ASSIGNMENTS
Lab 3: WRITE UP
Lab 4: Gibson assembly of HCAII into pETblue2 vector and transformation of E. coli with the reactions
Lab 4: BACKGROUND
Lab 4: PROCEDURE
Lab 4: LAB ASSIGNMENTS
Lab 4: MINI LAB REPORT
Lab 6: Mutant Exploration
Lab 6: BACKGROUND
Lab 6: PROCEDURE
Lab 6: LAB ASSIGNMENTS
Lab 6: MINI LAB REPORT
Lab 5: Screening for pETblue2-HCAII clones
Lab 5: BACKGROUND
Lab 5: PROCEDURE
Lab 5: LAB ASSIGNMENTS
Lab 5: MINI LAB REPORT
Lab 7: Protein expression and purification in E. coli
Lab 7: BACKGROUND
Lab 7: PROCEDURE
Lab 7: LAB ASSIGNMENTS
Lab 7: MINI LAB REPORT
Lab 8: Analysis of His-tagged HCAII expression and purification
Lab 8: BACKGROUND
Lab 8: PROCEDURE
Lab 8: LAB ASSIGNMENTS
Lab 8: DATA ANALYSIS
Lab 8: MINI LAB REPORT
Lab 9: Determination of Protein Stability using Chemical Denaturation and Intrinsic Fluorescence
Lab 9: AT-HOME EXPERIMENT
Lab 9: BACKGROUND
Lab 9: PROCEDURE
Lab 9: LAB ASSIGNMENTS
Lab 9: DATA ANALYSIS
Lab 9 MINI LAB REPORT
Lab 10: Investigation of the wild type and mutant HCAII enzyme activity
Lab 10: AT-HOME EXPERIMENT
Lab 10: BACKGROUND
Lab 10: PROCEDURE
Lab 10: LAB ASSIGNMENTS
Lab 10: DATA ANALYSIS
Lab 10: MINI LAB REPORT
Lab 11: FRET to detect ligand binding to HCAII
Lab 11: BACKGROUND
Lab 11: PROCOTOL
Lab 11: LAB ASSIGNMENTS
Lab 11: DATA ANALYSIS
Lab 11: FINAL LAB REPORT
Appendix 1: Information on HCAII
Appendix 2: Statistical methods used in 551 data analyses
Appendix 3: Additional laboratory details
Appendix 4: Buffer formulations
Appendix 5: Operation of the Shimadzu spectrophotometers
Appendix
Lab 11 Objectives
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Biochemistry 551 (Online Version) Lab Manual Copyright © by Lynne PROST is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.