Lab 6: LAB ASSIGNMENTS

PRE-LAB QUIZ

This week’s quiz is based on the mutageneic primers needed for the QuikChange PCR. You will be given a mutation number and the primers that were used to generate the mutation. Based on this information, determine the specific amino acid change for your mutation.

IN-LAB ACTIVITIES

You and your partner will meet in a BBCU breakout room to work through the lab procedure together.

• Work through the lab procedure and the provided Google doc, which ask you to:
  • create a protocol for QuikChange mutagenesis
  • complete a sequence alignment
  • generate a PyMOL figure
  • discuss what makes a good hypothesis and critique several sample hypotheses
  • write your own hypothesis
• Put the required information into your slides in the shared class Google slides presentation.
• At the designated time, you will join a TA and several other pairs to present and discuss your slides.
• Complete the lab/partner/self evaluation Google form.
• Get a sharable link to the Google doc and submit it to Canvas.