Lab 8: Analysis of His-tagged HCAII expression and purification

Lab 8 Objectives

  • Use SDS-PAGE to:
    • Determine expression of HCAII protein was induced in E. coli
    • Determine whether HCAII was successfully purified on a nickel column
  • Measure the protein concentration of the final purified HCAII protein
Fig. 8.1. A sample SDS-PAGE gel.

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Biochemistry 551 (Online Version) Lab Manual Copyright © by Lynne PROST is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.

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